Two MSAs were performed using Clustal Omega software. One was carried out between Homo Sapiens STAT5B and other related proteins within the STAT family. The other was carried out between STAT5B proteins across different species.
The results are shown in a boxshade. This format visualises the similarities between sequences by highlighting. Black highlighting means the amino acid residue is shared among most species, grey highlighting shows that those residues share similar chemical properties, and no highlight means the amino acids are unrelated.
The Boxshade was annotated to add further information. Red-lettered residues indicate conserved phosphorylation sites. Phosphorylation sites were found using the PhosphoELM, Scansite and NetworKIN programs.The domains were found using the Pfam domain search. The lines indicating each domain are colour coded according to the figure below.
Results from Pfam domain search.
Figure showing domain colours.
The Boxshade of this MSA shows that almost all residues are highlighted in black. This indicates that the sequence of STAT5B is highly conserved among species.
Homo Sapiens STAT family
STAT5B across species
